โ† Lab Calculator Free ยท No sign up ยท Instant

๐Ÿงช Serial Dilution Calculator

Calculate concentrations across each step of a serial dilution. Ideal for standard curve preparation and microbiological assays.

per step
Step Dilution Concentration Volume to Transfer Diluent to Add
๐Ÿ’ก Tip: For a 1:2 dilution enter factor 2. For 1:10 enter 10. For 2-fold dilution enter 2. "Volume per Tube" is the total final volume in each tube โ€” transfer the listed amount from the previous tube, then top up with diluent to reach that volume.
โš ๏ธ Verify before use: This calculator is a convenience aid. Double-check critical dilutions independently before use in experiments, especially for sensitive reagents or assays.
๐Ÿ“– Example: To prepare a 1:2 serial dilution series from 1 mg/mL stock in 1 mL tubes (transfer 0.5 mL each step): enter Stock = 1 mg/mL, Dilution Factor = 2, Tube Volume = 1 mL, Steps = 5. The table gives you tube-by-tube concentrations and volumes. Use "Copy Table" to paste into Excel or your lab notebook.
๐Ÿ“Š

Standard curves

Perfect for preparing 6-point or 8-point calibration curves for HPLC and ELISA.

๐Ÿ”ข

Up to 20 steps

Calculate serial dilutions across up to 20 steps in one go.

๐Ÿ†“

Always free

No sign up, no limits. Use as many times as you need.

โœ” Formulas last verified against primary references: March 2025  ยท  Report an error
โš ๏ธ Report an incorrect calculation
Try next โ†’
๐Ÿ’ง Simple Dilution Calculator๐Ÿ”ฌ Purity & Potency Calculator

Serial dilution in quantitative biology and microbiology

Serial dilution is used wherever concentrations must span several orders of magnitude: standard curves for ELISA and HPLC, MIC assays, CFU counting, and receptor binding studies. Each tube is diluted by the same factor, so concentrations follow a geometric series. The transfer volume = tube volume รท dilution factor. Use this calculator to plan your dilution scheme before bench work and to check that your chosen factor gives concentrations in the detection range of your assay.