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💧 Simple Dilution Calculator

Calculate any unknown variable using the C1V1 = C2V2 equation. Enter three values to find the fourth.

Solve for:

C₁ — Stock Concentration

V₁ — Stock Volume to Take

C₂ — Desired Concentration

V₂ — Final Volume Needed

Result

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💡 Important: C₁ and C₂ must be in the same concentration units (they're locked together automatically). V₁ and V₂ can be entered in different volume units (μL/mL/L) — the calculator converts between them automatically.

⚠️ Verify before use: This calculator is a convenience aid. Double-check critical dilutions independently before use in experiments, especially for sensitive reagents or assays.

📖 Example: You have a 10 mg/mL stock solution and need 1 mL of 0.5 mg/mL working solution. Set Solve for = V₁, enter C₁ = 10 mg/mL, C₂ = 0.5 mg/mL, V₂ = 1 mL → answer: take 0.05 mL (50 µL) of stock, add 0.95 mL diluent. Paste this result into your lab notebook or thesis Methods section.

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Any units

Works with mg/mL, μg/mL, molarity, % w/v and more. Keep C and V units consistent.

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Solve any variable

Solve for any of the four variables — C₁, V₁, C₂ or V₂.

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Always free

No sign up, no limits. Calculate as many dilutions as you need.

✔ Formulas last verified against primary references: March 2025 · Report an error

⚠️ Report an incorrect calculation

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🧪 Serial Dilution Calculator ⚖️ Concentration Converter

Simple dilution (C₁V₁ = C₂V₂) in the lab

A dilution calculator applies the conservation equation C₁V₁ = C₂V₂ — the amount of solute is constant when you dilute. Enter any three of the four variables to find the fourth. Useful for preparing working solutions from stock, adjusting assay concentrations, or diluting samples before measurement. Always verify units are consistent: keep volumes in the same unit (µL or mL) and concentrations in the same unit (mg/mL, mol/L) on both sides. A common error is confusing final volume V₂ with the diluent volume to add — the diluent volume = V₂ − V₁. For thesis reporting: state the stock concentration, working concentration, diluent, and final volume. Example: '50 µL of stock (10 mg/mL) was diluted with 950 µL PBS to give a 0.5 mg/mL working solution (1:20, v/v).'